PECULIARITIES OF MALE GAMETOPHYTE
DEVELOPMENT AS A BASIS FOR GENETICS AND SELECTION ESTIMATION OF ABIES SIBIRICA
LEDEB. TREES
Bazhina E.V., Kvitko
O.V.
V.N. Sukachev Institute
of Forest Siberian branch of
Artificial populations of conifers
are founded mainly from seeds. Tree breeding for seeds getting is based on habitus and descendants. But seed quality depends on gametes
forming at micro- and macrosporogenesis.
The aim of this work is analysis of
male gametophyte development and pollen quality for genetics and selection
estimation of Abies sibirica
Ledeb. trees.
Male strobili
were collected from healthy and drying fir trees in
The researches were shown that pattern of male gametophyte
development in Abies sibirica
healthy and drying trees
was found to be largely
the same as in other conifers species. Matured pollen grains of Abies sibirica have 4 cells: two
degenerated prothallial cell, generative and
vegetative cells. There is a tendency to decreasing of pollen size in drying
trees.
Pollen
irregularities were found in 0.03-0.77% healthy and 0.04-0.56% drying trees.
There are small and gigantic pollen grains in all trees. In drying trees there
are also abnormal pollen forms: grains with emergences and lens form. All trees
have pollen sacs abnormalities: collar and no equal on the size. Besides, in
healthy tree pollen without sacs and with 3 of them was marked. In drying trees
there were pollen grains with 4 pollen sacs. Analysis of pollen morphology is
no show statistical proved differences of abnormalities frequency between
healthy and drying trees (table), it not exceed 1%. However there are trees
with mainly sterile pollen (abnormal - 0.56-0.77%).
Frequency of abnormalities in Abies sibirica
pollen
|
Indexes |
Healthy |
Drying |
|
Quantity of analysed pollen grains |
11698 |
7915 |
|
Frequency of abnormalities, % |
0,61±0,10 |
0,77±0,14 |
Thus, the analysis of Abies sibirica male gametophyte
and pollen development allow to estimate of tree for breeding not only habitus and genetics polymorphism but with male development
peculiarities.
The work
was financial support with